Regulatory

Part:BBa_K3350859:Design

Designed by: Hao Xu and Yanqiang Xi   Group: iGEM20_NEFU_China   (2020-10-20)


yqjF1st (promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

To improve the sensitivity of the yqjF promoter to DNT for its practical application, we conducted semi-rational mutagenesis in its -35 region and generated its mutated version, the yqjF1st promoter. This mutagenesis decreased the DNT detection threshold from 25 mg/L of the original yqjF promoter to 15 mg/L of the yqjF1st promoter, which is a double fold increase of the sensitivity approximately.


References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.